ARG81826

Rat N Cadherin ELISA Kit

Rat N Cadherin ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81826-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81826-002 Standard 2 X 50 ng/vial 4°C
ARG81826-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81826-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81826-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81826-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81826-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81826-008 25X Wash buffer 20 ml 4°C
ARG81826-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81826-010 STOP solution 10 ml (Ready to use) 4°C
ARG81826-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81826 Rat N Cadherin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat N Cadherin in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name N Cadherin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.5%; Inter-Assay CV: 5.0%
Alternate Names Neural cadherin; N-cadherin; CDw325; CDHN; CD antigen CD325; NCAD; Cadherin-2; CD325

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 83501 Rat CDH2

Swiss-port # Q9Z1Y3 Rat Cadherin-2

Gene Symbol CDH2
Gene Full Name cadherin 2, type 1, N-cadherin (neuronal)
Background N Cadherin is a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
Function N Cadherin is a calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. [UniProt]
Highlight Related products:
Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).

May be phosphorylated by OBSCN. [UniProt]
Title Download Link
ARG81826 Rat N Cadherin ELISA Kit User's manual download icon Download