ARG81665

Rat Neurotrophin 3 ELISA Kit

Rat Neurotrophin 3 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81665-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81665-002 Standard 2 X 10 ng/vial 4°C
ARG81665-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81665-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81665-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81665-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81665-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81665-008 25X Wash buffer 20 ml 4°C
ARG81665-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81665-010 STOP solution 10 ml (Ready to use) 4°C
ARG81665-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81665 Rat Neurotrophin 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Neurotrophin 3 in serum and cell culture supernatants.
Tested Reactivity Rat
Predict Reactivity Bov, Chk, Dog, Hrs, Mk, Rb
Tested Application ELISA
Specificity There is cross-reactivity with NT-4 < 2.5%, and no detectable cross-reactivity with any other cytokine.
Target Name Neurotrophin 3
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 7.8 pg/ml
Sample Type Serum and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.9%; Inter-Assay CV: 7.3%
Alternate Names Nerve growth factor 2; NT3; HDNF; Neurotrophic factor; Neurotrophin-3; NT-3; NGF2; NGF-2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81737 Rat NTF3

Swiss-port # P18280 Rat Neurotrophin-3

Gene Symbol NTF3
Gene Full Name neurotrophin 3
Background The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]
Function Seems to promote the survival of visceral and proprioceptive sensory neurons. [UniProt]
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Title Download Link
ARG81665 Rat Neurotrophin 3 ELISA Kit User's manual download icon Download