ARG82572

Rat R Cadherin / Cadherin 4 ELISA Kit

Rat R Cadherin / Cadherin 4 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82572-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82572-002 Standard 2 X 10 ng/vial 4°C
ARG82572-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82572-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82572-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82572-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82572-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82572-008 25X Wash buffer 20 ml 4°C
ARG82572-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82572-010 STOP solution 10 ml (Ready to use) 4°C
ARG82572-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82572 Rat R Cadherin / Cadherin 4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat R Cadherin / Cadherin 4 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name R Cadherin / Cadherin 4
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.2%
Inter-Assay CV: 7.1%
Alternate Names R-CAD; Cadherin-4; Retinal cadherin; CAD4; RCAD; R-cadherin

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CDH4
Gene Full Name cadherin 4, type 1, R-cadherin (retinal)
Background This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. [UniProt]
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Title Download Link
ARG82572 Rat Cadherin 4 ELISA Kit User manual download icon Download