ARG82382
Rat Semaphorin 3F ELISA Kit
Rat Semaphorin 3F ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82382-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82382-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82382-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82382-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82382-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82382-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82382-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82382-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82382-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82382-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82382-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82382 Rat Semaphorin 3F ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Semaphorin 3F in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | Semaphorin 3F |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.1% Inter-Assay CV: 6.2% |
| Alternate Names | Sema III/F; SEMA-IV; Semaphorin IV; SEMA4; SEMAK; Sema IV; Semaphorin-3F |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SEMA3F |
|---|---|
| Gene Full Name | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F |
| Background | The semaphorins are a family of proteins that are involved in signaling. All the family members have a secretion signal, a 500-amino acid sema domain, and 16 conserved cysteine residues (Kolodkin et al., 1993 [PubMed 8269517]). Sequence comparisons have grouped the secreted semaphorins into 3 general classes, all of which also have an immunoglobulin domain. The semaphorin III family, consisting of human semaphorin III (SEMA3A; MIM 603961), chicken collapsin, and mouse semaphorins A, D, and E, all have a basic domain at the C terminus. Chicken collapsin contributes to path finding by axons during development by inhibiting extension of growth cones (Luo et al., 1993 [PubMed 8402908]) through an interaction with a collapsin response mediator protein of relative molecular mass 62K (CRMP62) (Goshima et al., 1995 [PubMed 7637782]), a putative homolog of an axonal guidance associated UNC33 gene product (MIM 601168). SEMA3F is a secreted member of the semaphorin III family.[supplied by OMIM, Mar 2008] |
| Function | May play a role in cell motility and cell adhesion. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: Semaphorin antibodies; Semaphorin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82382 Rat Semaphorin 3F ELISA Kit User manual |
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