ARG82540
Rat Semaphorin 4G ELISA Kit
Rat Semaphorin 4G ELISA Kit for ELISA and Rat
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82540-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82540-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82540-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82540-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82540-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82540-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82540-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82540-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82540-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82540-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82540-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82540 Rat Semaphorin 4G ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Semaphorin 4G in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Target Name | Semaphorin 4G |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 10 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 6.5% |
| Alternate Names | Semaphorin-4G |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | SEMA4G |
|---|---|
| Gene Full Name | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4G |
| Background | Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| Function | Cell surface receptor for PLXNB2. May play a role in axon guidance (By similarity). [UniProt] |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| Highlight | Related products: Semaphorin antibodies; Semaphorin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Title | Download Link |
|---|---|
| ARG82540 Rat Semaphorin 4G ELISA Kit User manual |
 Download
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