ARG82831

Rat Serpin D1 / Heparin cofactor II ELISA Kit

Rat Serpin D1 / Heparin cofactor II ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82831-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82831-002 Standard 2 X 50 ng/vial 4°C
ARG82831-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82831-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82831-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82831-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82831-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82831-008 25X Wash buffer 20 ml 4°C
ARG82831-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82831-010 STOP solution 10 ml (Ready to use) 4°C
ARG82831-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82831 Rat Serpin D1 / Heparin cofactor II ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat Serpin D1 / Heparin cofactor II in serum, plasma (EDTA, citrate), cell culture supernatants and urine.
Tested Reactivity Rat
Tested Application ELISA
Target Name Serpin D1 / Heparin cofactor II
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 0.39 ng/ml
Sample Type Serum, plasma (EDTA, citrate), cell culture supernatants and urine.
Standard Range 0.78 - 50 ng/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.2%
Inter-Assay CV: 5.6%
Alternate Names HC2; Protease inhibitor leuserpin-2; D22S673; HC-II; HCII; THPH10; Heparin cofactor 2; Heparin cofactor II; HCF2; LS2; HLS2; Serpin D1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79224 Rat SERPIND1

Swiss-port # Q64268 Rat Heparin cofactor 2

Gene Symbol SERPIND1
Gene Full Name serpin peptidase inhibitor, clade D (heparin cofactor), member 1
Background This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015]
Function Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.

Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. [UniProt]
PTM Phosphorylated by FAM20C in the extracellular medium. [UniProt]
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