ARG81771

Rat TIM1 / KIM1 ELISA Kit

Rat TIM1 / KIM1 ELISA Kit for ELISA and Rat

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Component

Cat No Component Name Package Temp
ARG81771-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81771-002 Standard 2 X 10 ng/vial 4°C
ARG81771-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81771-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81771-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81771-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81771-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81771-008 25X Wash buffer 20 ml 4°C
ARG81771-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81771-010 STOP solution 10 ml (Ready to use) 4°C
ARG81771-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81771 Rat TIM1 / KIM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat TIM1 / KIM1 in serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name TIM1 / KIM1
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 15.6 pg/ml
Sample Type Serum, plasma (heparin, EDTA), urine and cell culture supernatants.
Standard Range 31.2 - 2000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 6.1%; Inter-Assay CV: 6.9%
Alternate Names KIM-1; TIM1; TIM-1; TIMD-1; HAVcr-1; KIM1; HAVCR; TIMD1; CD365; T-cell immunoglobulin and mucin domain-containing protein 1; TIM; HAVCR-1; T-cell immunoglobulin mucin receptor 1; T-cell membrane protein 1; Kidney injury molecule 1; Hepatitis A virus cellular receptor 1

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 286934 Rat HAVCR1

Swiss-port # O54947 Rat Hepatitis A virus cellular receptor 1 homolog

Gene Symbol HAVCR1
Gene Full Name hepatitis A virus cellular receptor 1
Background The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 4, 12 and 19. [provided by RefSeq, Apr 2015]
Function May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. [UniProt]
Highlight Related products:
TIM1 antibodies; TIM1 ELISA Kits; TIM1 Duos / Panels;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData

Specific References

Urinary metabolomics study of vancomycin-associated nephrotoxicity based on ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry

ELISA / Rat / Serum

Pei Cao  et al.
Hum Exp Toxicol.,  (2022)

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Urinary Metabolomics Study of Vancomycin-Associated Nephrotoxicity Based on UHPLC-QTOF-MS/MS

ELISA / Rat / Urine

Pei Cao et al.
SSRN,  (2022)

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Title Download Link
ARG81771 Rat TIM1 / KIM1 ELISA Kit User manual download icon Download