ARG81880

Rat TIMP2 ELISA Kit

Rat TIMP2 ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG81880-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81880-002 Standard 2 X 10 ng/vial 4°C
ARG81880-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81880-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81880-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81880-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81880-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81880-008 25X Wash buffer 20 ml 4°C
ARG81880-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81880-010 STOP solution 10 ml (Ready to use) 4°C
ARG81880-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG81880 Rat TIMP2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat TIMP2 in serum, plasma (heparin, EDTA) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Specificity There is no detectable cross-reactivity with other relevant proteins.
Target Name TIMP2
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 78 pg/ml
Sample Type Serum, plasma (heparin, EDTA) and cell culture supernatants.
Standard Range 156 - 10000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.7%; Inter-Assay CV: 6.8%
Alternate Names TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29543 Rat TIMP2

Swiss-port # P30121 Rat Metalloproteinase inhibitor 2

Gene Symbol TIMP2
Gene Full Name TIMP metallopeptidase inhibitor 2
Background This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt]
Highlight Related products:
TIMP2 antibodies; TIMP2 ELISA Kits;
Related news:
Detecting MMPs and their non-ECM substrates
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
PTM The activity of TIMP2 is dependent on the presence of disulfide bonds. [UniProt]
Title Download Link
ARG81880 Rat TIMP2 ELISA Kit User manual download icon Download