ARG70346

SARS-CoV-2 Envelope recombinant protein (Avi-His-tagged, N-ter)

SARS-CoV-2 Envelope recombinant protein (Avi-His-tagged, N-ter) for SDS-PAGE,Binding Activity and Virus

Overview

Product Description E. coli expressed, Avi-His-tagged (N-ter) SARS-CoV-2 Envelope recombinant protein.
Tested Reactivity Virus
Tested Application Binding, SDS-PAGE
Target Name SARS-CoV-2 Envelope protein
Species Virus
A.A. Sequence Met1 - Val75 of SARS-CoV-2 Envelope protein (QHD43418.1) with an initial Met at the N-terminus and Avi-6X His tag at the N-terminus.
Expression System E. coli

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Envelope at 2µg/ml (100 µl/well) can bind Recombinant SARS-CoV-2 Nucleocapsid with a linear range of 1.2-41.1 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer 20 mM Tris (pH 8.0), 250 mM NaCl and 0.5% TritonX-100.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.