ARG70343
SARS-CoV-2 Spike recombinant protein (RBD) (His-tagged, C-ter)
SARS-CoV-2 Spike recombinant protein (RBD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Virus
Overview
| Product Description | HEK293 expressed, His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (RBD). |
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| Tested Reactivity | Virus |
| Tested Application | Binding, SDS-PAGE |
| Target Name | SARS-CoV-2 Spike protein (RBD) |
| Species | Virus |
| A.A. Sequence | Arg319 - Phe541 of SARS-CoV-2 Spike protein (YP_009724390.1) with 6X His tag at the C-terminus. |
| Expression System | HEK293 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike RBD-His at 2µg/ml (100 µl/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/ml. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE); > 95% (by HPLC) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
