ARG70344
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (Fc-His-tagged, C-ter)
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (Fc-His-tagged, C-ter) for SDS-PAGE,Binding Activity and Virus
Overview
| Product Description | HEK293 expressed, Fc-His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (S1 Subunit). |
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| Tested Reactivity | Virus |
| Tested Application | Binding, SDS-PAGE |
| Target Name | SARS-CoV-2 Spike protein (S1 Subunit) |
| Species | Virus |
| A.A. Sequence | Val11 - Arg682 of SARS-CoV-2 Spike protein (S1 Subunit) (YP_009724390.1) with an Fc-6X His tag at the C-terminus. |
| Expression System | HEK293 |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2µg/ml (100 µl/well) can bind Recombinant SARS-CoV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/ml. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 90% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
