ARG70218
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter)
SARS-CoV-2 Spike recombinant protein (S1 Subunit) (His-tagged, C-ter) for SDS-PAGE and Virus
Publication11
Overview
| Product Description | E. coli expressed, His-tagged (C-ter) SARS-CoV-2 Spike recombinant protein (S1 Subunit). |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | SDS-PAGE |
| Target Name | SARS-CoV-2 Spike protein (S1 Subunit) |
| Species | Virus |
| A.A. Sequence | MVNLT TRTQL PPAYT NSFTR GVYYP DKVFR SSVLH STQDL FLPFF SNVTW FHAIH VSGTN GTKRF DNPVL PFNDG VYFAS TEKSN IIRGW IFGTT LDSKT QSLLI VNNAT NVVIK VCEFQ FCNDP FLGVY YHKNN KSWME SEFRV YSSAN NCTFE YVSQP FLMDL EGKQG NFKNL REFVF KNIDG YFKIY SKHTP INLVR DLPQG FSALE PLVDL PIGIN ITRFQ TLLAL HRSYL TPGDS SSGWT AGAAA YYVGY LQPRT FLLKY NENGT ITDAV DCALD PLSET KCTLK SFTVE KGIYQ TSNFR VQPTE SIVRF PNITN LCPFG EVFNA TRFAS VYAWN RKRIS NCVAD YSVLY NSASF STFKC YGVSP TKLND LCFTN VYADS FVIRG DEVRQ IAPGQ TGKIA DYNYK LPDDF TGCVI AWNSN NLDSK VGGNY NYLYR LFRKS NLKPF ERDIS TEIYQ AGSTP CNGVE GFNCY FPLQS YGFQP TNGVG YQPYR VVVLS FELLH APATV CGPKK STNLV KNKCV NFNFN GLTGT GVLTE SNKKF LPFQQ FGRDI ADTTD AVRDP QTLEI LDITP CSFGG VSVIT PGTNT SNQVA VLYQD VNCTE VPVAI HADQL TPTWR VYSTG SNVFQ TRAGC LIGAE HVNNS YECDI PIGAG IC with polyhistidine tag at the C-terminus. |
| Expression System | E. coli |
Properties
| Form | Powder |
|---|---|
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 98% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related products: SARS-CoV antibodies; SARS-CoV ELISA Kits; SARS-CoV recombinant proteins; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 Exploring Antiviral Immune Response |
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Specific References
Detoxified synthetic bacterial membrane vesicles as a vaccine platform against bacteria and SARS-CoV-2
Functional assay /
Cytokine-Induced iNOS in A549 Alveolar Epithelial Cells: A Potential Role in COVID-19 Lung Pathology
Functional assay /
SARS-CoV-2 Spike protein is not pro-inflammatory in human primary macrophages: endotoxin contamination and lack of protein glycosylation as possible confounders
Functional Assay / Virus
Immune-Mediated Inflammatory Responses of Alveolar Epithelial Cells: Implications for COVID-19 Lung Pathology
Functional assay /
In Vitro Exposure of Primary Human T Cells and Monocytes to Polyclonal Stimuli Reveals a Basal Susceptibility to Display an Impaired Cellular Immune Response and Develop Severe COVID-19
Cell Stimulation /
Growth Arrest of Alveolar Cells in Response to Cytokines from Spike S1-Activated Macrophages: Role of IFN-γ
Functional assay / Human
The JAK1/2 Inhibitor Baricitinib Mitigates the Spike-Induced Inflammatory Response of Immune and Endothelial Cells In Vitro
Standardized Extract of Asparagus officinalis Stem Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages
Functional assay / Virus
Endothelial Cell Activation by SARS-CoV-2 Spike S1 Protein: A Crosstalk between Endothelium and Innate Immune Cells
Stimulation /
ETAS®50 Attenuates SARS-CoV-2 Spike Protein-Induced IL-6 and IL-1β Production by Suppressing p44/42 MAPK and Akt Phosphorylation in Murine Primary Macrophages.
SARS-CoV-2 spike protein S1 subunit induces pro-inflammatory responses via toll-like receptor 4 signaling in murine and human macrophages
Stimulation /
