ARG70347

SARS-CoV-2 nucleocapsid recombinant protein (His-tagged, N-ter)

SARS-CoV-2 nucleocapsid recombinant protein (His-tagged, N-ter) for SDS-PAGE,ELISA,Binding Activity and Virus

Overview

Product Description E. coli expressed, His-tagged (N-ter) SARS-CoV-2 nucleocapsid recombinant protein.
Tested Reactivity Virus
Tested Application Binding, ELISA, SDS-PAGE
Target Name SARS-CoV-2 nucleocapsid protein
Species Virus
A.A. Sequence Met1 - Ala419 of SARS-CoV-2 nucleocapsid protein (QHD43423.2) with 6X His tag at the N-terminus.
Expression System E. coli

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 µg/ml (100 µl/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/ml.
Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 µg/ml (100 µl/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE); > 95% (by HPLC)
Buffer 20 mM Tris (pH 8.0), 500 mM NaCl, 0.1 mM EDTA and 10% Glycerol.
Stabilizer 10% Glycerol
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.