ARG70347
SARS-CoV-2 nucleocapsid recombinant protein (His-tagged, N-ter)
SARS-CoV-2 nucleocapsid recombinant protein (His-tagged, N-ter) for SDS-PAGE,ELISA,Binding Activity and Virus
Overview
| Product Description | E. coli expressed, His-tagged (N-ter) SARS-CoV-2 nucleocapsid recombinant protein. |
|---|---|
| Tested Reactivity | Virus |
| Tested Application | Binding, ELISA, SDS-PAGE |
| Target Name | SARS-CoV-2 nucleocapsid protein |
| Species | Virus |
| A.A. Sequence | Met1 - Ala419 of SARS-CoV-2 nucleocapsid protein (QHD43423.2) with 6X His tag at the N-terminus. |
| Expression System | E. coli |
Application Instructions
| Application Note | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 µg/ml (100 µl/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/ml. Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 µg/ml (100 µl/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/ml. |
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Properties
| Form | Powder |
|---|---|
| Purification Note | 0.22 µm filter sterilized. Endotoxin level is less than 1.0 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE); > 95% (by HPLC) |
| Buffer | 20 mM Tris (pH 8.0), 500 mM NaCl, 0.1 mM EDTA and 10% Glycerol. |
| Stabilizer | 10% Glycerol |
| Storage Instruction | Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
