ARG82192

Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric)

Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species

Overview

Product Description ARG82192 Sorbitol Dehydrogenase Activity Assay Kit (Colorimetric) can be used to measure Sorbitol Dehydrogenase activity in serum, plasma, urine, tissue and cell culture supernatants.
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name Sorbitol Dehydrogenase
Conjugation Note Read at 565 nm
Sensitivity 0.1 U/L
Detection Range 0.1 - 125 U/L
Sample Type serum, plasma, urine, tissue and cell culture supernatants
Sample Volume 20 µl
Alternate Names SORD1; L-iditol 2-dehydrogenase; Sorbitol dehydrogenase; HEL-S-95n; EC 1.1.1.14

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time 15 min

Properties

Form Liquid
Storage Instruction Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20322 Mouse SORD

GeneID: 24788 Rat SORD

GeneID: 6652 Human SORD

Gene Symbol SORD
Gene Full Name sorbitol dehydrogenase
Background Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
Function Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. [UniProt]
Cellular Localization Mitochondrion membrane; Peripheral membrane protein. Cell projection, cilium, flagellum. Note=Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. [UniProt]
Title Download Link
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