ARG81133

Sphingosine 1-Phosphate ELISA Kit

Sphingosine 1-Phosphate ELISA Kit for ELISA and Bovine,Goat,Horse,Human,Mouse,Rat

Overview

Product Description ARG81133 Sphingosine 1-Phosphate ELISA Kit is an Enzyme Immunoassay kit for the quantification of Sphingosine 1-Phosphate in serum, plasma, tissue homogenate and cell culture lysate.
Tested Reactivity Hu, Ms, Rat, Bov, Goat, Hrs
Tested Application ELISA
Target Name Sphingosine 1-Phosphate
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sample Type Serum, plasma, tissue homogenate and cell culture lysate
Standard Range 0.03 - 4 µM
Sample Volume Serum and Plasma Samples: 25 µl
Cell Lysate or Tissue Homogenate: 30 µg/well

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Sphingosine 1-Phosphate (S1P) is key component of the sphingolipid signaling cascade. S1P initiates a proliferative, pro-angiogenic and anti-apoptotic sequence of events contributing to cancer progression. Recently, scientific literature has suggested that S1P is a potent tumorigenic growth factor that is likely released from tumor cells and that S1P may be a novel biomarker for early stage cancer detection. Sphingosine kinase has also been shown to be up-regulated in a variety of cancer types (S1P is produced via the activity of sphingosine kinase phosphorylating sphingosine). 
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