ARG30062
Tag Internal Control Antibody Duo (YFP, GAPDH)
Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Publication36
Component
Cat No | Component Name | Host clonality | Reactivity | Application | Package |
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ARG10112 | anti-GAPDH antibody [6C5] | Mouse mAb | AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Hu, Mk, Ms, Pig, Rb, Rat, Xenopus laevis, Zfsh | ELISA, ICC/IF, IHC-Fr, WB | 50 μg |
ARG20304 | anti-YFP antibody | Rabbit pAb | Other | ICC/IF, IP, WB | 25 μg |
Overview
Product Description | Reporter genes (often simply reporter) are chosen as reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because they are selectable markers. The commonly used reporters including GFP, dsRed protein (RFP), YFP, β-galactosidaseis, luciferase an so on, could be used to analysis gene regulation, gene expression or as a tag to trace the location of the protein. To study the gene regulation by protein level, arigo recommend to include GAPDH as a loading control to normalize the sample loading and the experimental errors to show the convincing result. GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blot (35-40kDa) and ICC/IF study. Usage note: S-nitrosylated GAPDH might translocate to nucleus especially for nitric oxide-related studies. In addition, the GAPDH expression level is up regulated under hypoxia condition. Therefore, GAPDH is not suitable for loading control and cytoplasm marker for oxygen-related and nitric oxide-related studies. |
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Target Name | Tag Internal Control |
Alternate Names | Tag Internal Control antibody; GAPDH antibody; YFP antibody |
Properties
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Gene Full Name | Antibody Duo for Tag Internal Control (YFP, GAPDH) |
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Research Area | Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
Specific References