ARG30062

Tag Internal Control Antibody Duo (YFP, GAPDH)

Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication36

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG10112 anti-GAPDH antibody [6C5] Mouse mAb AGMK, Bb, Cat, Chk, Dog, Fsh, Hm, Hu, Mk, Ms, Pig, Rb, Rat, Xenopus laevis, Zfsh ELISA, ICC/IF, IHC-Fr, WB 50 μg
ARG20304 anti-YFP antibody Rabbit pAb Other ICC/IF, IP, WB 25 μg

Overview

Product Description Reporter genes (often simply reporter) are chosen as reporters because the characteristics they confer on organisms expressing them are easily identified and measured, or because they are selectable markers. The commonly used reporters including GFP, dsRed protein (RFP), YFP, β-galactosidaseis, luciferase an so on, could be used to analysis gene regulation, gene expression or as a tag to trace the location of the protein. To study the gene regulation by protein level, arigo recommend to include GAPDH as a loading control to normalize the sample loading and the experimental errors to show the convincing result. GAPDH is often stably and constitutively expressed at high levels in most tissues and cells in cytoplasm. Therefore, GAPDH is a widely used loading control and cytoplasm marker for western blot (35-40kDa) and ICC/IF study. Usage note: S-nitrosylated GAPDH might translocate to nucleus especially for nitric oxide-related studies. In addition, the GAPDH expression level is up regulated under hypoxia condition. Therefore, GAPDH is not suitable for loading control and cytoplasm marker for oxygen-related and nitric oxide-related studies.
Target Name Tag Internal Control
Alternate Names Tag Internal Control antibody; GAPDH antibody; YFP antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Duo for Tag Internal Control (YFP, GAPDH)
Research Area Cancer antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Specific References

Qi-Tai-Suan, an oleanolic acid derivative, ameliorates ischemic heart failure via suppression of cardiac apoptosis, inflammation and fibrosis

ARG10112: WB / Human

QIAN Ming et al.
 Chinese Journal of Natural Medicines ,  (2022)

publication_link

 

hr_line

SCM-198 Prevents Endometriosis by Reversing Low Autophagy of Endometrial Stromal Cell via Balancing ERα and PR Signals

ARG10112: WB / Mouse

Yi-Kong Lin  et al.
Front Endocrinol (Lausanne),  (2022)

publication_link

 

hr_line

Pharmacological inhibition of SMYD2 protects against cisplatin-induced acute kidney injury in mice

ARG10112: WB / Mouse

Binbin Cui  et al.
Front Pharmacol,  (2022)

publication_link

 

hr_line

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

ARG10112: WB / Human

Yuhua Zhou et al.
Cent Eur J Immunol .,  (2022)

publication_link

 

hr_line

Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model

ARG10112: WB / Pig

Jianni Huang et al.
Frontiers in Cell and Developmental Biology,  (2022)

publication_link

 

hr_line

Lack of interferon regulatory factor 3 leads to anxiety/depression-like behaviors through disrupting the balance of neuronal excitation and inhibition in mice

ARG10112: WB / Mouse

Junjie Li et al.
Genes & Diseases,  (2022)

publication_link

 

hr_line

Genetic labeling reveals cellular expression pattern of neuregulin 1 in mouse forebrain

ARG10112: WB / Human

Chen-Yun Ding et al.
Research square,  (2022)

publication_link

 

hr_line

M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA.

ARG10112: WB / Human, Mouse

Xinlei Hu et al.
Br J Pharmacol.,  (2021)

publication_link

 

hr_line

Curcumin attenuates renal ischemia reperfusion injury via JNK pathway with the involvement of p300/CBP-mediated histone acetylation

ARG10112: WB / Rat

Lu Yang et al.
Korean J Physiol Pharmacol.,  (2021)

publication_link

 

hr_line

7,8-dihydroxyflavone ameliorates motor deficits via regulating autophagy in MPTP-induced mouse model of Parkinson's disease

ARG10112: WB / Mouse

Li Zuo et al.
Cell Death Discov.,  (2021)

publication_link

 

hr_line

Cardiac-Specific Gene TNNI3 as a Potential Oncogene for Kidney Cancer and Its Involvement in Wnt Signaling Pathway.

ARG10112: WB / Human

Weian Zhao et al.
Preprint.,  (2021)

publication_link

 

hr_line

Excessive fibroblast growth factor 23 promotes renal fibrosis in mice with type 2 cardiorenal syndrome.

ARG10112: WB / Mouse, Rat

Huixin Hao et al.
Aging (Albany NY).,  (2021)

publication_link

 

hr_line

Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.

ARG10112: WB / Human

Yi-An Chen et al.
Biomedicines.,  (2021)

publication_link

 

hr_line

Synthesis and anti-inflammatory activity of saponin derivatives of δ-oleanolic acid

ARG10112: WB / Human

Liu Liu et al.
Eur J Med Chem .,  (2021)

publication_link

 

hr_line

Circ_0081572 inhibits the progression of periodontitis through regulating the miR-378h/RORA axis.

ARG10112: WB / Human

Jing Wang et al.
Arch Oral Biol.,  (2021)

publication_link

 

hr_line

Evaluation of Class IIa Histone Deacetylases Expression and In Vivo Epigenetic Imaging in a Transgenic Mouse Model of Alzheimer's Disease

ARG10112: WB / Human

Yi-An Chen et al.
Int J Mol Sci.,  (2021)

publication_link

 

hr_line

Structural Basis of VSIG3: The Ligand for VISTA.

ARG10112: WB / Human

Xiaoxue Xie et al.
Front Immunol.,  (2021)

publication_link

 

hr_line

The Phosphorylation Status of Drp1-Ser637 by PKA in Mitochondrial Fission Modulates Mitophagy via PINK1/Parkin to Exert Multipolar Spindles Assembly during Mitosis.

ARG10112: WB / Human

Huey-Jiun Ko et al.
Biomolecules.,  (2021)

publication_link

 

hr_line

Cardiac troponin I R193H mutant interacts with HDAC1 to repress phosphodiesterase 4D expression in cardiomyocytes

ARG10112: WB / Mouse

Weian Zhao et al.
Genes Dis.,  (2020)

publication_link

 

hr_line

ERK activation precedes Purkinje cell loss in mice with Spinocerebellar ataxia type 17.

ARG10112: WB / Mouse

Chia-Wei Lin et al.
Neurosci Lett.,  (2020)

publication_link

 

hr_line

Study of isolation and characterization of extracellular vesicles in senescent tumor cells induced by chemotherapeutics.

ARG10112: WB / Human

Qi Zhou et al.
Chinese Journal of Immunology.,  (2020)

publication_link

 

hr_line

SMARCB1 Promotes Ubiquitination and Degradation of NR4A3 via Direct Interaction Driven by ROS in Vascular Endothelial Cell Injury

ARG10112: WB / Human, Rat

Bingzheng Lu et al.
Oxid Med Cell Longev.,  (2020)

publication_link

 

hr_line

Discovery of AdipoRon analogues as novel AMPK activators without inhibiting mitochondrial complex I.

ARG10112: WB / Human, Mouse

Geng Sun et al.
Eur J Med Chem.,  (2020)

publication_link

 

hr_line

Increased expression levels of inflammatory cytokines and adhesion molecules in lipopolysaccharide‑induced acute inflammatory apoM‑/‑ mice

ARG10112: WB / Mouse

Yuanping Shi et al.
Mol Med Rep.,  (2020)

publication_link

 

hr_line

Tumor-associated neutrophils induce EMT by IL-17a to promote migration and invasion in gastric cancer cells.

ARG10112: WB / Human

Li Sen et al.
J Exp Clin Cancer Res.,  (2019)

publication_link

 

hr_line

Genetic labeling reveals temporal and spatial expression pattern of D2 dopamine receptor in rat forebrain.

ARG10112: WB / Rat

Yu Qing et al.
Brain Struct Funct.,  (2019)

publication_link

 

hr_line

SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways.

ARG10112: WB / Human

Yunyun Li et al.
Acta Biochim Biophys Sin.,  (2019)

publication_link

 

hr_line

Epigenetic regulation of phosphodiesterase 4d in restrictive cardiomyopathy mice with cTnI mutations.

ARG10112: WB / Mouse

Zhao Weian et al.
Sci China Life Sci.,  (2019)

publication_link

 

hr_line

AdipoRon prevents l-thyroxine or isoproterenol-induced cardiac hypertrophy through regulating the AMPK-related pathway.

ARG10112: WB / Rat

Hu Xinlei et al.
Acta Biochim Biophys Sin (Shanghai).,  (2018)

publication_link

 

hr_line

The effect of melatonin on cardio fibrosis in juvenile rats with pressure overload and deregulation of HDACs.

ARG10112: WB / Rat

Wu Yao et al.
Korean J Physiol Pharmacol.,  (2018)

publication_link

 

hr_line

Epigallocatechin-3 gallate prevents pressure overload-induced heart failure by up-regulating SERCA2a via histone acetylation modification in mice.

ARG10112: WB / Mouse

Liu Lifei et al.
PLoS One.,  (2018)

publication_link

 

hr_line

Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation

ARG10112: WB / Rat

Yao Wu et al.
BioMed Research International.,  (2017)

publication_link

 

hr_line

Checking transfer efficiency and equal loading via qualitative optical way in western blotting.

ARG10112: WB / Human

Gong JH et al.
Electrophoresis,  (2017)

publication_link

 

hr_line

Hydrogen sulfide protects against endoplasmic reticulum stress and mitochondrial injury in nucleus pulposus cells and ameliorates intervertebral disc degeneration.

ARG10112: WB / Human

Xu D et al.
Pharmacol Res.,  (2017)

publication_link

 

hr_line

Acute hyperglycemia suppresses left ventricular diastolic function and inhibits autophagic flux in mice under prohypertrophic stimulation.

ARG10112: WB / Mouse

Xie J et al.
Cardiovasc Diabetol.,  (2016)

publication_link

 

hr_line

Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.

ARG10112: WB / Human

Yao B et al.
Cell Signal.,  (2016)

publication_link

 

hr_line