ARG80816

Vitamin B12 ELISA Kit

Vitamin B12 ELISA Kit for ELISA and Other

Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit

Overview

Product Description ARG80816 Vitamin B12 ELISA Kit is a Enzyme Immunoassay kit for the quantification of Vitamin B12 in food (extraction, dilution).
Tested Reactivity Other
Tested Application ELISA
Target Name Vitamin B12
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 0.3 ng/ml
Sample Type Food (extraction, dilution).
Standard Range 0.4 - 40 ng/ml
Sample Volume 50 µl

Application Instructions

Assay Time 1 h (RT/shaker), 20 min (RT/dark)

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Vitamin B12
Background Vitamin B12 as a trace element belongs to the biologi­cally important chelate formers. The basic unit con­sists of a corrin ring with cobalt as a central atom. Cobalt is sixfold coordinated by four nitrogen atoms, one cyanide and a dimethylbenzimidazol group. Vitamin B12 forms a stable complex, which is absorbed in the lower part of the small intestine, with the so-called intrinsic factor present in the gastric juice. A lack of vitamin B12 can lead among other things to pernicious anemia. This disease is not generated by an insufficient supply of vitamin B12, but by the ab­sence of intrinsic factor. A pernicious anemia can be treated by a high dosage of vitamin B12.

The existing detection procedures are mainly microbiological methods, but also HPLC and thin-layer chromatography, all of which are associated with a high amount of time and instrumentation.

With the present test kit it is possible, to determine vitamin B12 quantitatively in vitaminated food in a significantly faster way (2.5 to 4 hours inclusive sam­ple pretreatment) compared with a conventional microbiological assay (24 to 48 hours).
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Research Area Cell Biology and Cellular Response kit; Metabolism kit; Signaling Transduction kit
Title Download Link
ARG80816 Vitamin B12 ELISA Kit User's manual download icon Download