ARG80817

Vitamin H (Biotin) ELISA Kit

Vitamin H (Biotin) ELISA Kit for ELISA and Other

Controls and Markers kit; Metabolism kit; Signaling Transduction kit

Overview

Product Description ARG80817 Vitamin H (Biotin) ELISA Kit is a Enzyme Immunoassay kit for the quantification of Vitamin H (Biotin) in food (extraction, dilution).
Tested Reactivity Other
Tested Application ELISA
Target Name Vitamin H
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 0.5 ng/ml
Sample Type Food (extraction, dilution).
Standard Range 1 - 25 ng/ml
Sample Volume 50 µl

Application Instructions

Assay Time 1 h (RT/shaker), 20 min (RT/dark)

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Vitamin H / Biotin
Background Biotin serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysin residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide.

The awareness of the population for a good health and its interest in healthy nutrition has increased sig­nificantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer.

When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. As biotin is synthesized by the human intestinal flora, deficiency symptoms are rare, appear however after excessive ingestion of raw egg white, which can be explained by its content of biotin-binding avidin.
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Research Area Controls and Markers kit; Metabolism kit; Signaling Transduction kit
Title Download Link
ARG80817 Vitamin H (Biotin) ELISA Kit User's manual download icon Download