ARG63881

anti-14-3-3 sigma / Stratifin antibody

anti-14-3-3 sigma / Stratifin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description

Goat Polyclonal antibody recognizes 14-3-3 sigma / Stratifin

Tested Reactivity Hu, Ms, Rat
Predict Reactivity Dog, Pig
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name 14-3-3 sigma / Stratifin
Antigen Species Human
Immunogen C-DLHTLSEDSYKDST
Conjugation Un-conjugated
Alternate Names YWHAS; 14-3-3 protein sigma; Epithelial cell marker protein 1; Stratifin

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
WB1:1000 - 1:2500
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2810 Human SFN

GeneID: 55948 Mouse SFN

Swiss-port # O70456 Mouse 14-3-3 protein sigma

Swiss-port # P31947 Human 14-3-3 protein sigma

Gene Symbol SFN
Gene Full Name stratifin
Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.
p53-regulated inhibitor of G2/M progression. [UniProt]
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Research Area Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 28 kDa
PTM Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation.

Customer's Feedback

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Review for anti-14-3-3 sigma / Stratifin antibody

Application:WB

Sample:Rat lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-14-3-3 sigma / Stratifin antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC