ARG64363

anti-14-3-3 tau / YWHAQ antibody

anti-14-3-3 tau / YWHAQ antibody for Western blot and Human

Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description Goat Polyclonal antibody recognizes 14-3-3 tau / YWHAQ
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name 14-3-3 tau / YWHAQ
Antigen Species Human
Immunogen C-DDRKQTIDNSQ
Conjugation Un-conjugated
Alternate Names 14-3-3; Protein HS1; 1C5; HS1; 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10971 Human YWHAQ

Swiss-port # P27348 Human 14-3-3 protein theta

Background This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]
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Research Area Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 28 kDa
PTM Ser-232 is probably phosphorylated by CK1.

Customer's Feedback

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Review for anti-14-3-3 tau / YWHAQ antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Average

Review for anti-14-3-3 tau / YWHAQ antibody

Application:WB

Sample:Mouse brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC