ARG51169
anti-14-3-3 zeta antibody
anti-14-3-3 zeta antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes 14-3-3 zeta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 14-3-3 zeta |
| Antigen Species | Human |
| Immunogen | Peptide sequence around aa.56~60 (R-S-S-W-R) derived from Human 14-3-3 zeta. |
| Conjugation | Un-conjugated |
| Alternate Names | HEL4; 14-3-3-zeta; Protein kinase C inhibitor protein 1; YWHAD; KCIP-1; HEL-S-3; 14-3-3 protein zeta/delta |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | YWHAZ |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta |
| Background | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| Function | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. [UniProt] |
| Highlight | Related products: 1433 zeta antibodies; 1433 zeta Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: 14-3-3η as a promising target for the treatment of Major Depression Disorder Has “Obesity gene” been found? |
| Research Area | Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 28 kDa |
| PTM | The delta, brain-specific form differs from the zeta form in being phosphorylated (By similarity). Phosphorylation on Ser-184 by MAPK8; promotes dissociation of BAX and translocation of BAX to mitochondria. Phosphorylation on Thr-232; inhibits binding of RAF1. Phosphorylated on Ser-58 by PKA and protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion. Phosphorylation on Ser-58 by PKA; disrupts homodimerization and heterodimerization with YHAE and TP53. |
