ARG23717

anti-4 Hydroxynonenal antibody [12F7]

anti-4 Hydroxynonenal antibody [12F7] for ELISA,ICC/IF,Western blot and Other

Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody
publication_link Publication7

Overview

Product Description Mouse Monoclonal antibody [12F7] recognizes 4 Hydroxynonenal
Tested Reactivity Other
Tested Application ELISA, ICC/IF, WB
Specificity Specific for 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxynonenal. Does not cross-react with 4-Hydroxy-2-hexenal, Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
Host Mouse
Clonality Monoclonal
Clone 12F7
Isotype IgG1
Target Name 4 Hydroxynonenal
Antigen Species Others
Immunogen Synthetic 4-Hydroxynonenal modified KLH.
Conjugation Un-conjugated
Alternate Names 4-HNE

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:1000
ICC/IF1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol.
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related Antibody Duos and Panels:
ARG30337 Ferroptosis / Oxytosis Antibody Panel
Related products:
Hydroxynonenal antibodies; Hydroxynonenal ELISA Kits; Hydroxynonenal Duos / Panels; Anti-Mouse IgG secondary antibodies;
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Research Area Ferroptosis/Oxytosis Study antibody; Lipid peroxidation Marker antibody

Specific References

Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism

WB / Mouse

Jixian Song et al.
Curr Issues Mol Biol.,  (2023)

publication_link

 

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FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice

IHC-P / Mouse

Lipeng Li et al.
Ecotoxicol Environ Saf.,  (2023)

publication_link

 

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A novel quinoline derivative, DFIQ, sensitizes NSCLC cells to ferroptosis by promoting oxidative stress accompanied by autophagic dysfunction and mitochondrial damage

WB / Human

Yung-Ding Bow et al.
Cancer Cell Int.,  (2023)

publication_link

 

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Effects of liraglutide on astrocyte polarization and neuroinflammation in db/db mice: focus on iron overload and oxidative stress

IHC-P / Mouse

Ji-Ren An et al.
Front Cell Neurosci.,  (2023)

publication_link

 

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Fermented and Aged Ginseng Sprouts ( Panax ginseng) and Their Main Component, Compound K, Alleviate Asthma Parameters in a Mouse Model of Allergic Asthma through Suppression of Inflammation, Apoptosis, ER Stress, and Ferroptosis

WB / Mouse

Ji Hyeon Ryu et al.
Antioxidants (Basel),  (2022)

publication_link

 

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Liraglutide attenuates hepatic iron levels and ferroptosis in db/db mice

WB / Mouse

Ji-Xian Song et al.
Bioengineered.,  (2022)

publication_link

 

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Oyster-Derived Tyr-Ala (YA) Peptide Prevents Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure by Suppressing Inflammatory, Apoptotic, Ferroptotic, and Pyroptotic Signals

WB / Mouse

Adrian S Siregar et al.
Mar Drugs.,  (2021)

publication_link

 

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