ARG63405

anti-4E-T / EIF4ENIF1 antibody

anti-4E-T / EIF4ENIF1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes 4E-T / EIF4ENIF1
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2; NP_001157973.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name 4E-T / EIF4ENIF1
Antigen Species Human
Immunogen C-AKVISVDELEYRQ
Conjugation Un-conjugated
Alternate Names 4E-T; Eukaryotic translation initiation factor 4E nuclear import factor 1; Eukaryotic translation initiation factor 4E transporter; eIF4E transporter; Clast4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 - 10 µg/ml
WB0.25 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56478 Human EIF4ENIF1

Swiss-port # Q9NRA8 Human Eukaryotic translation initiation factor 4E transporter

Background The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic; its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Research Area Gene Regulation antibody
Calculated MW 108 kDa