ARG52216
anti-5 Lipoxygenase phospho (Ser523) antibody
anti-5 Lipoxygenase phospho (Ser523) antibody for Western blot and Human,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes 5 Lipoxygenase phospho (Ser523) |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | NHuPrm |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | 5 Lipoxygenase |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | 5-lipoxygenase; Arachidonate 5-lipoxygenase; LOG5; 5-LO; EC 1.13.11.34; 5LPG; 5-LOX |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ALOX5 |
| Gene Full Name | arachidonate 5-lipoxygenase |
| Background | The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 78 kDa |
| PTM | Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. |
