ARG56116

anti-5-bromodeoxyuridine / BrdU antibody [BRD.3]

anti-5-bromodeoxyuridine / BrdU antibody [BRD.3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Other

Overview

Product Description Mouse Monoclonal antibody [BRD.3] recognizes 5-bromodeoxyuridine / BrdU
Tested Reactivity Other
Tested Application FACS, ICC/IF, IHC-P
Host Mouse
Clonality Monoclonal
Clone BRD.3
Isotype IgG1
Target Name 5-bromodeoxyuridine / BrdU
Immunogen Bromodeoxyuridine conjugated to BSA.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 min at RT followed by digestion with Trypsin at 1 mg/ml PBS, 10 min at 37°C.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Cellular Localization Nuclear