ARG57237

anti-5-hydroxymethylcytosine / 5-hmC antibody [RM236]

anti-5-hydroxymethylcytosine / 5-hmC antibody [RM236] for Dot blot,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,MeDIP and Other

Overview

Product Description

Rabbit Monoclonal antibody [RM236] recognizes 5-hydroxymethylcytosine / 5-hmC

Tested Reactivity Other
Tested Application Dot, ELISA, ICC/IF, IHC-P, MeDIP
Specificity This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
Host Rabbit
Clonality Monoclonal
Clone RM236
Isotype IgG
Target Name 5-hydroxymethylcytosine / 5-hmC
Antigen Species Others
Immunogen BSA-conjugated 5-hydroxymethylcytosine.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
Dot0.2 - 1 µg/ml
ELISA0.1 - 1 µg/ml
ICC/IF0.5 - 2 µg/ml
IHC-P0.1 - 1 µg/ml
MeDIP0.2 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol and 1% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.