ARG62337

anti-6X His tag antibody [HIS.H8]

anti-6X His tag antibody [HIS.H8] for Dot blot,Electrophoretic mobility shift assay,ELISA,ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody
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Overview

Product Description Mouse Monoclonal antibody [HIS.H8] recognizes 6X His tag
Tested Reactivity Other
Tested Application Dot, ELISA, EMSA, ICC/IF, IP, WB
Specificity Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6X His-tags)
Host Mouse
Clonality Monoclonal
Clone HIS.H8
Isotype IgG2b
Target Name 6X His tag
Antigen Species Others
Immunogen KLH-conjugated HHHHHH (6X His) synthetic peptide.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
DotAssay-dependent
ELISAAssay-dependent
EMSAAssay-dependent
ICC/IF1:500 - 1:2000
IP1:200
WB1:1000 - 1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A Purified
Purification Note Protein A affinity chromatography from mouse ascites fluid.
Buffer 10mM PBS (pH 7.2) and 0.05% Sodium azide.
Preservative 0.05% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
His-tagged protein is also useful for the scientitists for the binding assay and tracing the location of the protein-interested in the cells when the specific antibodies for the protein-of-interest are unavailable.
Highlight Related Antibody Duos and Panels:
ARG30002 Tag Internal Control Antibody Duo (His tag, GAPDH)
Related products:
6x His tag antibodies; 6x His tag ELISA Kits; 6x His tag Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Controls and Markers antibody; Tag Internal Control antibody

Customer's Feedback

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Review for anti-6X His tag antibody [HIS.H8]

Application:WB

Sample:The crude BL21 soluble fractions containing recombinant PA28α, PA28β, and PA28γ proteasome subunits

Sample Loading Amount:1 µg

Primary Antibody Dilution Factor:1:10000

Primary Antibody Incubation Time:1 hour

Primary Antibody Incubation Temperature:25 ºC

Clone References

Sentrin/SUMO specific proteases as novel tissue-selective modulators of vitamin D receptor-mediated signaling.

WB / 

Lee WP et al.
PLoS One.,  (2014)

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Protein interaction screening for the ankyrin repeats and suppressor of cytokine signaling (SOCS) box (ASB) family identify Asb11 as a novel endoplasmic reticulum resident ubiquitin ligase.

WB / 

Andresen CA et al.
J Biol Chem.,  (2014)

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Binding of G-quadruplexes to the N-terminal recognition domain of the RNA helicase associated with AU-rich element (RHAU).

IP / 

Meier M et al.
J Biol Chem.,  (2013)

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Smad6 inhibits non-canonical TGF-β1 signalling by recruiting the deubiquitinase A20 to TRAF6.

Jung SM et al.
Nat Commun.,  (2013)

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A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.

WB / Human

Cloutier P et al.
PLoS Genet.,  (2013)

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The embryonic transcription cofactor LBH is a direct target of the Wnt signaling pathway in epithelial development and in aggressive basal subtype breast cancers.

EMSA / 

Rieger ME et al.
Mol Cell Biol.,  (2010)

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Subcellular localization of CIAPIN1.

ICC/IF / 

Hao Z et al.
J Histochem Cytochem.,  (2006)

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