ARG52222

anti-ABCA4 antibody [3F4]

anti-ABCA4 antibody [3F4] for Western blot,Immunohistochemistry and Bovine,Human,Mouse,Xenopus laevis

Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [3F4] recognizes ABCA4
Tested Reactivity Hu, Ms, Bov, Xenopus laevis
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone 3F4
Isotype IgG
Target Name ABCA4
Antigen Species Bovine
Immunogen Partially purified bovine 220-kDa disc rim protein
Conjugation Un-conjugated
Alternate Names STGD; RMP; RIM protein; ABC10; Stargardt disease protein; Retinal-specific ATP-binding cassette transporter; ARMD2; ATP-binding cassette sub-family A member 4; RIM ABC transporter; FFM; RP19; ABCR; STGD1; CORD3; RmP

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:100
WB1:1000
Application Note Specific for ABCA4
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11304 Mouse ABCA4

GeneID: 24 Human ABCA4

GeneID: 281584 Bovine ABCA4

Background ABCA4 (ATP-binding cassette, sub-family A (ABC1), member 4, Rim Protein) is a member of the superfamily of ATP-binding cassette (ABC) transporters (Illing et al., 1997). ABC proteins transport various molecules across extra- and intracellular membranes. This protein is a retina-specific ABC transporter with N-retinylidene-PE as a substrate. It is expressed exclusively in retina photoreceptor cells, indicating the gene product mediates transport of an essential molecule across the photoreceptor cell membrane. Mutations in this gene are found in patients diagnosed with Stargardt disease and are associated with retinitis pigmentosa-19 and age-related macular degeneration (Wiszniewski et al., 2003). Defects in ABCA4 are the cause of Stargardt disease type 1 (STGD1) (Molday et al., 2000). STGD is one of the most frequent causes of macular degeneration in childhood. Defects in ABCA4 are also known to cause fundus flavimaculatus (FFM), age-related macular degeneration type 2 (ARMD2) and cone-rod dystrophy type 3 (CORD3) (Klevering et al., 2005).
Research Area Neuroscience antibody
Calculated MW 256 kDa