ARG40299

anti-ABCF1 / ABC50 antibody

anti-ABCF1 / ABC50 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ABCF1 / ABC50
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ABCF1 / ABC50
Antigen Species Human
Immunogen Synthetic peptide derived from Human ABCF1.
Conjugation Un-conjugated
Alternate Names TNF-alpha-stimulated ABC protein; ATP-binding cassette sub-family F member 1; ABC50; ABC27; ATP-binding cassette 50

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4),0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23 Human ABCF1

Swiss-port # Q8NE71 Human ATP-binding cassette sub-family F member 1

Gene Symbol ABCF1
Gene Full Name ATP-binding cassette, sub-family F (GCN20), member 1
Background The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008]
Function Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis. [UniProt]
Cellular Localization Isoform 2: Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope. [UniProt]
Calculated MW 96 kDa
PTM Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes. [UniProt]