ARG62665

anti-ABRA1 / CCDC98 antibody [ABRA1-01]

anti-ABRA1 / CCDC98 antibody [ABRA1-01] for Flow cytometry,Immunoprecipitation,Western blot and Human

Gene Regulation antibody

Overview

Product Description

Mouse Monoclonal antibody [ABRA1-01] recognizes ABRA1 / CCDC98

Tested Reactivity Hu
Tested Application FACS, IP, WB
Specificity The clone ABRA1-01 recognizes N-terminal part of ABRA1 (Abraxas, CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kDa band on PAAGE under reducing conditions.
Host Mouse
Clonality Monoclonal
Clone ABRA1-01
Isotype IgG
Target Name ABRA1 / CCDC98
Immunogen Recombinant protein corresponding to amino acids 1-313 of ABRA1 with N-terminal His6 tag
Conjugation Un-conjugated
Alternate Names Protein FAM175A; Coiled-coil domain-containing protein 98; ABRA1; CCDC98; BRCA1-A complex subunit Abraxas

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note WB: Under reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 84142 Human FAM175A

Swiss-port # Q6UWZ7 Human BRCA1-A complex subunit Abraxas

Gene Symbol FAM175A
Gene Full Name family with sequence similarity 175, member A
Background ABRA1 (Abraxas), also known as CCDC98, is an adaptor protein that is essential for formation and function of BRCA1 A tumor suppressor complex. This complex plays critical roles in DNA repair, cell cycle checkpoint control, and maintenance of genomic stability. ABRA1 mediates interaction of ubiquitin-interacting motif-containing protein RAP80 and deubiquitination enzyme BRCC36 with BRCA1/BARD1. ABRA1 controls both DNA-damage-induced formation of BRCA1 foci and BRCA1-dependent G2/M checkpoint activation.
Function Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 47 kDa
PTM Phosphorylation of Ser-406 of the pSXXF motif by ATM or ATR constitutes a specific recognition motif for the BRCT domain of BRCA1 (PubMed:17643121, PubMed:17525340, PubMed:17643122). Ionizing radiation promotes rapid phosphorylation at Ser-404 and Ser-406 by ATM; this promotes recruitment of BRCA1 to sites of DNA damage (PubMed:26778126).