ARG57118

anti-ACAT1 antibody [15E5]

anti-ACAT1 antibody [15E5] for ICC/IF,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [15E5] recognizes ACAT1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 15E5
Isotype IgG1, kappa
Target Name ACAT1
Antigen Species Human
Immunogen Recombinant fragment around aa. 34-427 of Human ACAT1
Conjugation Un-conjugated
Alternate Names EC 2.3.1.9; Acetoacetyl-CoA thiolase; MAT; T2; ACAT; THIL; Acetyl-CoA acetyltransferase, mitochondrial

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 38 Human ACAT1

Swiss-port # P24752 Human Acetyl-CoA acetyltransferase, mitochondrial

Gene Symbol ACAT1
Gene Full Name acetyl-CoA acetyltransferase 1
Background This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]
Function Plays a major role in ketone body metabolism. [UniProt]
Calculated MW 45 kDa
PTM Succinylation at Lys-268, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5 (By similarity).