ARG41098

anti-ACE antibody

anti-ACE antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,IHC-Frozen sections,ICC/IF and Human,Mouse,Rat

publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes ACE
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ACE
Antigen Species Human
Immunogen Recombinant protein corresponding to K651-Y864 Human ACE.
Conjugation Un-conjugated
Alternate Names DCP1; ICH; ACE; EC 3.2.1.-; MVCD3; Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; CD143; CD antigen CD143; EC 3.4.15.1; Kininase II; ACE1; DCP

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 180 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11421 Mouse ACE

GeneID: 1636 Human ACE

GeneID: 24310 Rat ACE

Gene Symbol ACE
Gene Full Name angiotensin I converting enzyme
Background This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
Function Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. [UniProt]
Cellular Localization Angiotensin-converting enzyme, soluble form: Secreted. Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Detected in both cell membrane and cytoplasm in neurons. [UniProt]
Calculated MW 150 kDa
PTM Phosphorylated by CK2 on Ser-1299; which allows membrane retention. [UniProt]

Specific References

Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization

WB / Mouse

Jia-Xin Li et al.
Redox Biol.,  (2023)

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