ARG54913
anti-ACE2 antibody
anti-ACE2 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACE2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Bat, Cat, Frt |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Anti-ACE2 has no cross response to ACE1. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACE2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide within the last 50 aa of Human ACE2 (CKGENNPGFQNTDDVQTSF). |
| Conjugation | Un-conjugated |
| Alternate Names | Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 3.4.17.23 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 105 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACE2 |
| Gene Full Name | angiotensin I converting enzyme 2 |
| Background | ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function. |
| Function | Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses. [UniProt] |
| Highlight | Related products: ACE2 antibodies; ACE2 ELISA Kits; ACE2 recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1, a biomarker and therapeutic target in COVID-19 ACE2, receptor of 2019-nCoV |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 92 kDa |
| PTM | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. |
Specific References
Myeloid ACE2 protects against septic hypotension and vascular dysfunction through Ang-(1-7)-Mas-mediated macrophage polarization
IHC-Fr / Mouse
Antcins from Antrodia cinnamomea and Antrodia salmonea Inhibit Angiotensin-Converting Enzyme 2 (ACE2) in Epithelial Cells: Can Be Potential Candidates for the Development of SARS-CoV-2 Prophylactic Agents
WB / Human
Geranium and Lemon Essential Oils and Their Active Compounds Downregulate Angiotensin-Converting Enzyme 2 (ACE2), a SARS-CoV-2 Spike Receptor-Binding Domain, in Epithelial Cells.
WB / Human
