ARG65169

anti-ACO2 / Aconitase 2 antibody

anti-ACO2 / Aconitase 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ACO2 / Aconitase 2
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application ICC/IF, IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ACO2 / Aconitase 2
Antigen Species Human
Immunogen C-QHVDVSPTSQRLQ
Conjugation Un-conjugated
Alternate Names Aconitase; ACONM; EC 4.2.1.3; ICRD; Aconitate hydratase, mitochondrial; Citrate hydro-lyase; OCA8; HEL-S-284

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay - dependent
IHC-P3 - 5 µg/ml
WB0.01 - 0.03 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 50 Human ACO2

Swiss-port # Q99798 Human Aconitate hydratase, mitochondrial

Background The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq, Jul 2008]
Highlight Related products:
Aconitase antibodies; Aconitase ELISA Kits; Anti-Goat IgG secondary antibodies;
Related news:
TCA intermediate fumarate promotes mitobiogenesis
Research Area Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 85 kDa