ARG59829
anti-ACOT8 antibody
anti-ACOT8 antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ACOT8 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ACOT8 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-220 of Human ACOT8 (NP_005460.2). |
| Conjugation | Un-conjugated |
| Alternate Names | hACTE-III; hTE; Choloyl-coenzyme A thioesterase; EC 3.1.2.27; Peroxisomal acyl-coenzyme A thioester hydrolase 1; HNAACTE; PTE1; PTE2; Peroxisomal long-chain acyl-CoA thioesterase 1; PTE-2; PTE-1; Thioesterase II; hACTEIII; HIV-Nef-associated acyl-CoA thioesterase; Acyl-CoA thioesterase 8; Acyl-coenzyme A thioesterase 8 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa and Jurkat | ||||
| Observed Size | 36 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACOT8 |
| Gene Full Name | acyl-CoA thioesterase 8 |
| Background | The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010] |
| Function | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May mediate Nef-induced down-regulation of CD4. Major thioesterase in peroxisomes. Competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (By similarity). May be involved in the metabolic regulation of peroxisome proliferation. [UniProt] |
| Cellular Localization | Cytoplasm. Peroxisome matrix. Note=Predominantly localized in the peroxisome (PubMed:10092594, PubMed:15194431). [UniProt] |
| Calculated MW | 36 kDa |
