ARG63483

anti-ACSL4 / FACL4 antibody

anti-ACSL4 / FACL4 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ACSL4 / FACL4
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application FACS, ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise isoform 1 (NP_004449.1) and isoform 2 (NP_075266.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ACSL4 / FACL4
Antigen Species Human
Immunogen C-HYLKDIERMYGGK
Conjugation Un-conjugated
Alternate Names Long-chain-fatty-acid--CoA ligase 4; ACS4; MRX68; Long-chain acyl-CoA synthetase 4; EC 6.2.1.3; FACL4; LACS4; LACS 4; MRX63

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2182 Human ACSL4

Swiss-port # O60488 Human Long-chain-fatty-acid--CoA ligase 4

Background The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 79 kDa