ARG63960

anti-ACSL5 antibody

anti-ACSL5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ACSL5
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ACSL5
Antigen Species Human
Immunogen C-RTQIDSLYEHIQD
Conjugation Un-conjugated
Alternate Names FACL5; ACS5; Long-chain-fatty-acid--CoA ligase 5; ACS2; Long-chain acyl-CoA synthetase 5; EC 6.2.1.3; LACS 5

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51703 Human ACSL5

Swiss-port # Q9ULC5 Human Long-chain-fatty-acid--CoA ligase 5

Background The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 76 kDa