ARG54334
anti-ADAM10 / KUZ / MADM antibody
anti-ADAM10 / KUZ / MADM antibody for Western blot,ICC/IF and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ADAM10 / KUZ / MADM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody recognizes human, mouse, and rat ADAM10. A band of 85kDa is detected in immunoblots and may represent precursor. Lower mw bands are detected in some cell lines, including Jurkat, and may represent the processed mature protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ADAM10 / KUZ / MADM |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 732-748 of human ADAM10. This sequence is identical to those of bovine and rat and differs from mouse by one amino acid |
| Conjugation | Un-conjugated |
| Alternate Names | Kuzbanian protein homolog; kuz; HsT18717; MADM; RAK; EC 3.4.24.81; ADAM 10; AD18; CDw156; Mammalian disintegrin-metalloprotease; CD antigen CD156c; AD10; Disintegrin and metalloproteinase domain-containing protein 10; CD156c |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Jurkat and K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O14672 Human Disintegrin and metalloproteinase domain-containing protein 10 |
|---|---|
| Gene Symbol | ADAM10 |
| Gene Full Name | ADAM metallopeptidase domain 10 |
| Background | TNF , Notch and its ligand delta are all membrane-bound molecules which are cleaved by proteases to release mature proteins or functional receptors. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease) was recently found to cleave TNF , Notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 have been cloned and designated ADAM10, Kuzbanian (KUZ), and MADM, respectively. These genes are expressed in a variety of human and bovine tissues. |
| Function | Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | The precursor is cleaved by a furin endopeptidase. |
