ARG63730

anti-ADAM12 antibody

anti-ADAM12 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ADAM12
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Specificity This antibody is expected to recognise both the longer membrane-bound form of human ADAM12 (NP_003465.3) and the shorter soluble ADAM12 splice isoform (NP_067673.2). This antibody does not cross-react with other ADAMS.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ADAM12
Antigen Species Human
Immunogen AARPLPVSPARALC
Conjugation Un-conjugated
Alternate Names MCMP; ADAM 12; MCMPMltna; EC 3.4.24.-; Disintegrin and metalloproteinase domain-containing protein 12; Meltrin-alpha; MLTN; MLTNA; CAR10; ADAM12-OT1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
ICC/IF10 µg/ml
IHC-P3 - 5 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8038 Human ADAM12

Swiss-port # O43184 Human Disintegrin and metalloproteinase domain-containing protein 12

Background This gene encodes a member of the ADAM (a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 100 kDa
PTM The precursor is cleaved by a furin endopeptidase.