ARG66278
anti-ADAM17 / TACE phospho (Thr735) antibody
anti-ADAM17 / TACE phospho (Thr735) antibody for Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ADAM17 / TACE phospho (Thr735) |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | WB |
| Specificity | The antibody detects endogenous level of ADAM17 only when phosphorylated at Threonine 735. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ADAM17 / TACE |
| Antigen Species | Human |
| Immunogen | KLH-conjugated phospho-specific peptide around Thr735 (PQT(p)PG) of Human ADAM17. |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD156b; TNF-alpha convertase; NISBD; ADAM 17; Disintegrin and metalloproteinase domain-containing protein 17; Snake venom-like protease; TACE; NISBD1; ADAM18; EC 3.4.24.86; CSVP; CD156B; TNF-alpha-converting enzyme |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P78536 Human Disintegrin and metalloproteinase domain-containing protein 17 |
|---|---|
| Gene Symbol | ADAM17 |
| Gene Full Name | ADAM metallopeptidase domain 17 |
| Background | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008] |
| Function | Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. [UniProt] |
| Calculated MW | 93 kDa |
| PTM | The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at THR-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding. [UniProt] |
