ARG43613

anti-ADAM9 antibody

anti-ADAM9 antibody for Flow cytometry,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit polyclonal antibody recognizes ADAM9
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ADAM9
Antigen Species Human
Immunogen Recombinant fragment protein of Human ADAM9.
Conjugation Un-conjugated
Protein Full Name Disintegrin and metalloproteinase domain-containing protein 9
Alternate Names MDC9; Meltrin-gamma; ADAM 9; Metalloprotease/disintegrin/cysteine-rich protein 9; Disintegrin and metalloproteinase domain-containing protein 9; Cellular disintegrin-related protein; EC 3.4.24.-; CORD9; Mltng; MCMP; Myeloma cell metalloproteinase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
WB0.25 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431, A549, U251, U2OS whole cell lysates; Mouse, rat liver
Observed Size ~ 70-80 kDa (mature form), 100-115 kDa (pro-form)

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11502 Mouse ADAM9

GeneID: 8754 Human ADAM9

Swiss-port # Q13443 Human Disintegrin and metalloproteinase domain-containing protein 9

Swiss-port # Q61072 Mouse Disintegrin and metalloproteinase domain-containing protein 9

Gene Symbol ADAM9
Gene Full Name ADAM metallopeptidase domain 9
Background This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
Function Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. [UniProt]
Cellular Localization Cell membrane, Membrane, Secreted
Calculated MW 90.5 kDa
PTM Proteolytically cleaved in the trans-Golgi network before it reaches the plasma membrane to generate a mature protein. The removal of the pro-domain occurs via cleavage at two different sites. Processed most likely by a pro-protein convertase such as furin, at the boundary between the pro-domain and the catalytic domain. An additional upstream cleavage pro-protein convertase site (Arg-56/Glu-57) has an important role in the activation of ADAM9.

Phosphorylation is induced in vitro by phorbol-12-myristate-13-acetate (PMA). [UniProt]