ARG59548

anti-ADAMTS13 antibody

anti-ADAMTS13 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ADAMTS13
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ADAMTS13
Antigen Species Human
Immunogen Recombinant protein corresponding to A299-R488 of Human ADAMTS13.
Conjugation Un-conjugated
Alternate Names von Willebrand factor-cleaving protease; vWF-CP; EC 3.4.24.87; ADAM-TS 13; VWFCP; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS13; vWF-cleaving protease; ADAMTS-13; C9orf8

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11093 Human ADAMTS13

GeneID: 279028 Mouse ADAMTS13

Swiss-port # Q769J6 Mouse A disintegrin and metalloproteinase with thrombospondin motifs 13

Swiss-port # Q76LX8 Human A disintegrin and metalloproteinase with thrombospondin motifs 13

Gene Symbol ADAMTS13
Gene Full Name ADAM metallopeptidase with thrombospondin type 1 motif, 13
Background This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Function Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. [UniProt]
Cellular Localization Secreted. Note=Secretion enhanced by O-fucosylation of TSP type-1 repeats. [UniProt]
Calculated MW 154 kDa
PTM Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion.

The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. [UniProt]