ARG23679
anti-ADAMTS5 antibody
anti-ADAMTS5 antibody for Western blot,ELISA and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ADAMTS5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | The antibody reacts primarily with ADAMTS5. When ADAMTS5 and ADAMTS4 are analyzed with the antibody in Western blots and ELISA, ADAMTS5 is detected at 50-fold lower concentrations than ADAMTS4. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ADAMTS5 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated peptide derived from the hinge region between ADAMTS5 disintegrin- and thrombospondin-like domains. |
| Conjugation | Un-conjugated |
| Alternate Names | ADAMTS-5; EC 3.4.24.-; Aggrecanase-2; ADAM-TS 11; A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAMTS11; A disintegrin and metalloproteinase with thrombospondin motifs 11; ADAM-TS5; ADMP-2; ADAMTS-11; ADAM-TS 5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. The IgG fraction was purified by affinity chromatography. |
| Buffer | PBS (pH 7.5), 20 mM Na-phosphate, 150 mM NaCl and 0.02% Thimerosal. |
| Preservative | 0.02% Thimerosal |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9UNA0 Human A disintegrin and metalloproteinase with thrombospondin motifs 5 |
|---|---|
| Gene Symbol | ADAMTS5 |
| Gene Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 5 |
| Background | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. [provided by RefSeq, Jul 2008] |
| Function | Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. [UniProt] |
| Calculated MW | 102 kDa |
| PTM | The precursor is cleaved by a furin endopeptidase. C- and O-glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation can mediate the efficient secretion of ADAMTS family members. Can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). [UniProt] |
