ARG53742
anti-AGR2 + AGR3 antibody [AGR3.4]
anti-AGR2 + AGR3 antibody [AGR3.4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [AGR3.4] recognizes AGR2 + AGR3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | The clone AGR3.4 recognizes the PLMII epitope of AGR3 (AG3) and AGR2 (AG2) proteins (19-20 kDa); secreted cytoplasmic proteins which can serve as markers of carcinogenesis. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | AGR3.4 |
| Isotype | IgG1 |
| Target Name | AGR2 + AGR3 |
| Antigen Species | Human |
| Immunogen | Purified human AGR3 protein |
| Conjugation | Un-conjugated |
| Alternate Names | Anterior gradient protein 2 homolog; Secreted cement gland protein XAG-2 homolog; hAG-2; GOB-4; HAG-2; HPC8; PDIA17; HEL-S-116; AG-2; XAG-2; AG2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | WB and ICC/IF: Positive control: T47D. Negative control: H1299 IHC-P: Positive control: Human colon |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95994 Human Anterior gradient protein 2 homolog |
|---|---|
| Gene Symbol | AGR2 |
| Gene Full Name | anterior gradient 2 |
| Background | AGR2 (Anterior Gradient 2), also known as AG2 (hAG-2, HAG2 in human), or GOB-4, and AGR3 (Anterior Gradient 3), also known as AG3 (hAG-3, HAG3 in human), or BCMP11, are secreted cytoplasmic proteins which are involved in metastasis induction and p53 tumour supressor inhibition. They may serve as molecular markers and potential therapeutic targets for hormone-responsive breast tumours; AGR2 was reported also as a marker of other carcinomas. Xenopus homolog of these proteins is associated with anteroposterior fate determination during early development. |
| Function | Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 20 kDa |
