ARG62667
anti-AHNAK1 antibody [EM-09]
anti-AHNAK1 antibody [EM-09] for ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [EM-09] recognizes AHNAK1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-Fr, IP, WB |
| Specificity | The clone EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EM-09 |
| Isotype | IgG1 |
| Target Name | AHNAK1 |
| Antigen Species | Human |
| Immunogen | Bacterially expressed fragment of N-terminal domain of human AHNAK1. |
| Conjugation | Un-conjugated |
| Alternate Names | Desmoyokin; AHNAKRS; Neuroblast differentiation-associated protein AHNAK |
Application Instructions
| Application Suggestion |
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| Application Note | ICC/IF: Permeabilization is required. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB and ICC/IF: HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q09666 Human Neuroblast differentiation-associated protein AHNAK |
|---|---|
| Gene Symbol | AHNAK |
| Gene Full Name | AHNAK nucleoprotein |
| Background | AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex. |
| Function | May be required for neuronal cell differentiation. [UniProt] |
| Research Area | Cancer antibody; Signaling Transduction antibody |
| Calculated MW | 629 kDa |
