ARG23560
anti-AID antibody [EK2-5G9]
anti-AID antibody [EK2-5G9] for ELISA,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rat Monoclonal antibody [EK2-5G9] recognizes AID. Rat anti Human AID antibody, clone EK2-5G9 recognizes human Single-stranded DNA cytosine deaminase, also known as Activation-induced cytidine deaminase or Cytidine aminohydrolase. AID is a 198 amino acid ~24 kDa RNA-editing enzyme essential for the initiation of immunoglobulin class switch recombination, somatic hypermutation and gene conversion.AID is selectively expressed by germinal centre B cells and B cell-derived lymphomas (Basso et al. 2012). Mutation in the AICDA gene is responsible for the autosomal recessive condition Immunodeficiency with hyper-IgM 2 (HIGM2) A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE resulting in extreme susceptibility to bacterial infection. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, FACS, IHC-P, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | EK2-5G9 |
| Isotype | IgG2b |
| Target Name | AID |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around aa. 185-198 of Human AID. |
| Conjugation | Un-conjugated |
| Alternate Names | Cytidine aminohydrolase; CDA2; HIGM2; Activation-induced cytidine deaminase; AID; EC 3.5.4.38; ARP2; Single-stranded DNA cytosine deaminase; HEL-S-284 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in EDTA (pH 8.0). FACS: Membrane permeabilisation is required for this application. Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9GZX7 Human Single-stranded DNA cytosine deaminase |
|---|---|
| Gene Symbol | AICDA |
| Gene Full Name | activation-induced cytidine deaminase |
| Background | This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009] |
| Function | Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. [UniProt] |
| Calculated MW | 24 kDa |
| PTM | Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity. Probably monoubiquitinated on several residues by RNF126. [UniProt] |
