ARG59068

anti-AIF1 / Iba 1 antibody

anti-AIF1 / Iba 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes AIF1 / Iba 1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AIF1 / Iba 1
Antigen Species Human
Immunogen Synthetic peptide derived from Human AIF1 / Iba 1.
Conjugation Un-conjugated
Alternate Names Ionized calcium-binding adapter molecule 1; Allograft inflammatory factor 1; IBA1; IRT-1; IRT1; AIF-1; Protein G1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:100 - 1:500
IP1:50
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 16 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11629 Mouse AIF1

GeneID: 199 Human AIF1

GeneID: 29427 Rat AIF1

Gene Symbol AIF1
Gene Full Name allograft inflammatory factor 1
Background AIF1 / Iba 1 is a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]
Function AIF1 / Iba 1 is an Actin-binding protein. It enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, phagocytic cup. Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Activated Macrophage/Microglia Study antibody; Neuroinflammation Study antibody; Macroglial Marker antibody
Calculated MW 17 kDa
PTM Phosphorylated on serine residues. [UniProt]