ARG42926

anti-AIMP2 antibody

anti-AIMP2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes AIMP2
Tested Reactivity Hu, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AIMP2
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 298-320 of Human AIMP2. (NVQRWMRSCENLAPFNTALKLLK)
Conjugation Un-conjugated
Alternate Names PRO0992; JTV-1; JTV1; P38; Protein JTV-1; Multisynthase complex auxiliary component p38; Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 36 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 288480 Rat AIMP2

GeneID: 7965 Human AIMP2

Swiss-port # Q13155 Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Swiss-port # Q32PX2 Rat Aminoacyl tRNA synthase complex-interacting multifunctional protein 2

Gene Symbol AIMP2
Gene Full Name aminoacyl tRNA synthetase complex-interacting multifunctional protein 2
Background The protein encoded by this gene is part of the aminoacyl-tRNA synthetase complex, which contains nine different aminoacyl-tRNA synthetases and three non-enzymatic factors. The encoded protein is one of the non-enzymatic factors and is required for assembly and stability of the complex. [provided by RefSeq, May 2016]
Function Required for assembly and stability of the aminoacyl-tRNA synthase complex (PubMed:19131329). Mediates ubiquitination and degradation of FUBP1, a transcriptional activator of MYC, leading to MYC down-regulation which is required for aveolar type II cell differentiation. Blocks MDM2-mediated ubiquitination and degradation of p53/TP53. Functions as a proapoptotic factor. [UniProt]
Cellular Localization Cytoplasm, cytosol. Nucleus. Note=Following DNA damage, dissociates from the aminoacyl-tRNA synthase complex and translocates from the cytoplasm to the nucleus. [UniProt]
Calculated MW 35 kDa
PTM Phosphorylated on serine residues in response to UV irradiation.

Ubiquitinated by PRKN, leading to its degradation by the proteasome. Mutant PRKN fails to ubiquitinate AIMP2 efficiently, allowing its accumulation which may contribute to neurodegeneration associated with Parkinson disease. [UniProt]