ARG11086
anti-AKAP12 / Gravin antibody
anti-AKAP12 / Gravin antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Rat
Overview
| Product Description | Sheep Polyclonal antibody recognizes AKAP12 / Gravin |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | IHC-P, IP, WB |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | AKAP12 / Gravin |
| Antigen Species | Rat |
| Immunogen | Recombinant protein corresponding to aa. 620-1596 of Rat AKAP12 / Gravin. |
| Conjugation | Un-conjugated |
| Alternate Names | SSeCKS; AKAP250; A-kinase anchor protein 250 kDa; Gravin; A-kinase anchor protein 12; Myasthenia gravis autoantigen; AKAP 250; AKAP-12 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Rat aortic smooth muscle |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulfate precipitation. |
| Buffer | PBS and 0.08% Sodium azide. |
| Preservative | 0.08% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AKAP12 |
| Gene Full Name | A kinase (PRKA) anchor protein 12 |
| Background | The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Function | Anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). [UniProt] |
| Cellular Localization | Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Membrane; Lipid-anchor. Note=May be part of the cortical cytoskeleton. [UniProt] |
| Calculated MW | 181 kDa (Rat) |
