ARG59788

anti-ALKBH1 antibody

anti-ALKBH1 antibody for Western blot,Immunoprecipitation and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ALKBH1
Tested Reactivity Hu
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ALKBH1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 110-389 of Human ALKBH1 (NP_006011.2).
Conjugation Un-conjugated
Alternate Names DNA lyase ABH1; EC 4.2.99.18; ABH; Alkylated DNA repair protein alkB homolog 1; DNA oxidative demethylase ALKBH1; alkB; hABH; ABH1; Alpha-ketoglutarate-dependent dioxygenase ABH1; ALKBH; EC 1.14.11.33

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 293T and HT-29
Observed Size 44 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8846 Human ALKBH1

Swiss-port # Q13686 Human DNA oxidative demethylase ALKBH1

Gene Symbol ALKBH1
Gene Full Name alkB homolog 1, histone H2A dioxygenase
Background This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008]
Function Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. [UniProt]
Cellular Localization Nucleus. Mitochondrion. Note=Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli (By similarity). [UniProt]
Calculated MW 44 kDa